蔡海波

我校生物反应器工程国家重点实验室 教授，工学博士

生物化工专业：博士生导师/硕士生导师

生物工程专业：博士生导师/硕士生导师

生物与医药专业（生物工程领域）：博士生导师/硕士生导师

研究方向：

聚焦细胞治疗产业的需求，以干细胞以及免疫效应细胞为研究对象，开展基于生物反应器的干细胞以及免疫细胞体外扩增技术的研究，解决细胞治疗级效应细胞规模化制备过程中的关键工程学问题。

1.   研究体外培养过程中干细胞以及效应细胞的代谢特性，从信号通路及细胞代谢调控的角度，设计开发可满足相应细胞高效扩增的无血清培养基；

2.   研究体内微环境组分对干细胞体外扩增及分化的影响及作用机制，研究仿生微环境构建技术与方法，设计研制可模拟体内微环境的生物反应器系统以及过程工艺；

3.   研究高效的效应细胞体外规模化制备技术；

4.    研制细胞体外规模化扩增、冻存相关生物材料。

主持或参加的科研项目或基金：

主持有关干细胞生物工程相关的国家课题以及细胞治疗相关的企业课题。

教学：

承担生物工程专业《代谢调控》和生物技术专业《发酵工程》本科教学工作

近年代表性论文（^*通讯作者）：  

1. Jin Wang, Xiaodi Shi, Minghao Xiong, Wen-Song Tan, Haibo Cai*. Trehalose glycopolymers for cryopreservation of tissue-engineered constructs. Cryobiology, 2022, 104: 47-55.
2. Jin Wang, Minghao Xiong, Qihao Sun, Wen-Song Tan, Haibo Cai*. Threedimension co-culture of hematopoietic stem cells and differentiated osteoblasts on gallic acid grafted-chitosan scaffold as a model of hematopoietic stem cells niche. Stem Cell Reviews and Reports, 2022, 18: 1168-1180.
3. Jin Wang, Wubo He, Wen-Song Tan, Haibo Cai*. The chitosan / carboxymethyl cellulose / montmorillonite scaffolds incorporated with epigallocatechin-3-gallate loaded chitosan microspheres for promoting osteogenesis of human umbilical cord-derived mesenchymal stem cell. Bioresources and Bioprocessing, 2022, 9:36-50.
4. Yangyang Liu, Qihao Sun, Mengyang Hao,Wen‑Song Tan, Haibo Cai*. A novel magnetically controlled bioreactor for ex vivo expansion of NK-92 cells. Bioresources and Bioprocessing 2022， DOI: 10.1186/s40643-022-00537-z.
5. Huimin Huang, Zizhen Gong, Xuejun Zhu, Wen‐song Tan, Haibo Cai*. Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum-free medium. Biotechnology Progres 2022, DOI: 10.1002/btpr.3279
6. Qihao Sun, Yiran Zhou, Minghao Xiong, Yuying Chen, Wen-Song Tan, Haibo Cai*. MEK1 activation enhances the ex vivo proliferation of hematopoietic stem/progenitor cell. Cell Biochemistry and Function, 2022, 1-11. doi:10.1002/cbf.3677
7. Xuejun Zhu, Qihao Sun, Wen-song Tan, Haibo Cai *. Removal of CD34- cells to increase self-renewal symmetric division and expansion ex vivo of cord blood CD34+ cells through reducing the TGF-β1. Process Biochemistry, 2022, 112: 209-216

8. Xuejun Zhu, Qihao Sun, Wen-song Tan， Haibo Cai*. Reducing TGF-β1 cooperated with StemRegenin 1 promoted the expansion ex vivo of cord blood CD34+ cells by inhibiting AhR signalling. Cell Proliferation, 2021, DOI: 10.1111/cpr.12999
9. Jin Wang, Qihao Sun, Yin Wei, Mengyang Hao, Wen-Song Tan, Haibo Cai⁎. Sustained release of epigallocatechin-3-gallate from chitosan-based scaffolds to promote osteogenesis of mesenchymal stem cell. International Journal of Biological Macromolecules, 2021, 176: 96-105
10. Qihao Sun, Yan Fu, Xuejun Zhu, Wen-Song Tan, Haibo Cai*. Continuous NF-κB pathway inhibition promotes expansion of human phenotypical hematopoietic stem/progenitor cells through metabolism regulation. Experimental Cell Research, 2021, 399( 2): 112468
11. Qihao Sun, Yiran Zhou, Xuejun Zhu, Wen-Song Tan, and Haibo Cai*. Optimizing BIO feeding strategy promotes ex vivo expansion of human hematopoietic stem and progenitor cells. Journal of Bioscience and Bioengineering, 2021,131(2): 190-197
12. Mengyang Hao, Minghao Xiong, Yangyang Liu, Wen-song Tan and Haibo Cai*. Magnetic-driven dynamic culture promotes osteogenesis of mesenchymal stem cell. Bioresources and Bioprocessing, 2021,8:15
13. JinWang, LehaoZhou, QihaoSun, HaiboCai*, Wen-SongTan. Porous chitosan derivative scaffolds affect proliferation and osteogenesis of mesenchymal stem cell via reducing intracellular ROS. Carbohydrate Polymers, 2020, 237: 116108
14. Zhang Weiwei, Huang Huimin, Cai Haibo*, Tan Wen-Song. Enhanced metabolic activities for ATP production and elevated metabolic flux via pentose phosphate pathway contribute for better CIK cells expansion. Cell Proliferation. 2019, 52(3):e12594
15. Tang Chaochun, Zhang Weiwei, Cai Haibo*, Ye Zhaoyang, Zhang Xu, Tan Wensong. Resveratrol improves ex vivo expansion of CB-CD34+ cells via downregulating intracellular reactive oxygen species level. Journal of Cellular Biochemistry. 2019, 120(5):7778-7787
16. Zhang Yuanhao, Tong Yudong, Pan Xiuwei, Cai Haibo*, Gao Yun*, Zhang Weian*.Promoted Proliferation of Hematopoietic Stem Cells Enabled by a Hyaluronic Acid/Carbon Nanotubes Antioxidant Hydrogel.Macromolecular Materials and Engineering. 2019, 304(4): 1800630
17. Tong Zhang, Huanyun Chen, Yiran Zhou, Wanghong Dong, Haibo Cai*， WenSong Tan. Cooperation of FGF/MEK/ERK and Wnt/β-catenin pathway regulators to promote the proliferation and pluripotency of mouse embryonic stem cells in serum- and feeder-free conditions. Bioresources and Bioprocessing, 2019, 6(1):12
18. Zhang Weiwei, Cai Haibo*, Tan Wen-song.Dynamic suspension culture improves ex vivo expansion of cytokineinduced killer cells by upregulating cell activation and glucose consumption rate. Journal of Biotechnology, 2018, 287:8-17
19. Tong Yudong, Zhang Yuanhao, Liu Yangyang, Cai, Haibo*, Zhang Weian*, Tan Wen-Song. POSS-enhanced thermosensitive hybrid hydrogels for cell adhesion and detachment. RSC Advances , 2018, 8 (25) :13813-13819
20. Wenyan Zhang, Weiwei Zhang, Xu Zhang, Qin Lu, Haibo Cai*, Wen-Song Tan.Hyperoside promotes ex vivo expansion of hematopoietic stem/progenitor cells derived from cord blood by reducing intracellular ROS level. Process Biochemistry, 2018, 72:143-151
21. Yuanhao Zhang, Xiuwei Pan, Zhen Shi, Haibo Cai*, Yun Gao, Weian Zhang*. Sustained release of stem cell factor in a double network hydrogel for exvivo culture of cord blood‐derived CD34+ cells. Cell Proliferation, 2018,51(2):e12407
22. 刘洋洋，周乐豪，郝梦洋， 蔡海波*，谭文松,磁性搅拌珠制备及在 NK-92 细胞体外扩增中的应用, 高校化学工程学报, 2021,35(4): 683-689
23. 田连华，张 童， 蔡海波*，谭文松, 抗坏血酸对动态培养体系中 mESCs 扩增特性的影响, 生物学杂志, 2021,38(3):38-42
24. 应丹妮, 陈焕芸, 付岩, 蔡海波*, 谭文松, 谷氨酰胺和丙酮酸钠对 NK-92 细胞体外扩增的影响, 高校化学工程学报, 2020,34(3):728-736
25. 陈焕芸, 应丹妮, 蔡海波*, 谭文松, γ-聚谷氨酸在细胞冻存中的作用, 高分子通报, 2020(04):30-36
26. 龚子祯, 蔡海波*, 谭文松, 黄原胶支持 CIK 细胞高密度扩增, 高校化学工程学报, 2019,33(1):128-133
27. 卢意, 蔡海波*, 谭文松, 甘露糖和葡萄糖醛酸组合促进 CIK 细胞体外扩增, 中国免疫学杂志， 2019,35(15):1865-1870

发明专利：

1.蔡海波，陈小东，谭文松，张伟伟，施镇,用于免疫杀伤细胞体外扩增的血清替代物组合, ZL201610030317.9
2. 蔡海波，张伟伟，谭文松,细胞因子诱导杀伤细胞动态悬浮培养的方法,
ZL201611217280.7
3. 蔡海波，谭文松，陈小东，周乐豪, 用于人免疫杀伤细胞体外扩增的无血清培养基, ZL201710055741.3
4. 蔡海波，张伟安，童煜栋，谭文松，刘洋洋, 耐高温灭菌的细胞培养用水凝胶及其制备方法, ZL 20191012689.2
5. 蔡海波，陈焕芸，周乐豪，谭文松, 细胞冻存液, ZL201810902354.3
6. 蔡海波，童煜栋，张伟安，张远豪，谭文松，耐高温温敏型细胞培养介质材料及其制备方法， ZL 201810140945.1
7.蔡海波，王进，谭文松，负载EGCG纳米颗粒的壳聚糖-海藻酸钠复合支架及其制备方法，ZL202110168835.8
8. 蔡海波，刘洋洋，周乐豪，谭文松,用于细胞体外培养的磁控生物反应器系统 CN201910743602.9
9. 蔡海波，王进，何武博，谭文松，一种用于骨组织工程的壳聚糖复合缓释支架及其制备方法， 202110179886.0

联系方式：
通信地址：上海市梅陇路 130 号我校 309 信箱，邮编： 200237
实验室地址：徐汇校区实验 18 楼 1011 室
电话： 021-64251962
E-mail： caihaibo@ecust.edu.cn